Coding

Part:BBa_K4268001:Experience

Designed by: J. Aubrey, J. Alvarenga, L. Buchanan, J. Reyes, D. Yashinski   Group: iGEM22_SUNY_Oneonta   (2022-07-26)


The part was received, resuspended, and cloned into the level 0 Golden Gate vector, PSB1C00. The ligation was then transformed into DH5α cells. After overnight growth, white colonies (indicative of a replacement of the RFP reporter cassette) were subjected to screening for the correct insert using colony PCR with the VF/VR primer pair.


Figure 1: Colony PCR of five colonies (clones) suspected of containing the Capsid Assembly Protein insert. The insert length is 768bp and the predicted size of the PCR product when using VF/VR primers is 1073 bp.

The gel indicates that colonies 1 and 2 are likely to contain the correct insert and thus, the Capsid Assembly Protein was successfully cloned into a Level 0 Golden Gate Assembly basic part.


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